2013
Huang, K. -Y.; Shin, J. -W.; Huang, P. -J.; Ku, F. -M.; Lin, W. -C.; Lin, R.; Hsu, W. -M.; Tang, P.
Functional profiling of the Tritrichomonas foetus transcriptome and proteome Journal Article
In: Molecular and Biochemical Parasitology, 187 (1), pp. 60-71, 2013, ISSN: 01666851, (cited By 12).
Abstract | Links | BibTeX | 標籤: article; expressed sequence tag; matrix assisted laser desorption ionization time of flight mass spectrometry; nonhuman; priority journal; protein domain; protein expression; protein motif; ribosome; Tritrichomonas foetus; two dimensional electrophoresis, complementary DNA; proteome; transcriptome, Electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcriptome; Tritrichomonas foetus, Two-Dimensional; Expressed Sequence Tags; Proteome; Protozoan Proteins; Spectrometry
@article{Huang201360,
title = {Functional profiling of the Tritrichomonas foetus transcriptome and proteome},
author = {K. -Y. Huang and J. -W. Shin and P. -J. Huang and F. -M. Ku and W. -C. Lin and R. Lin and W. -M. Hsu and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873300752&doi=10.1016%2fj.molbiopara.2012.12.001&partnerID=40&md5=3d7c67beac54bc68da0563a156eb09ea},
doi = {10.1016/j.molbiopara.2012.12.001},
issn = {01666851},
year = {2013},
date = {2013-01-01},
journal = {Molecular and Biochemical Parasitology},
volume = {187},
number = {1},
pages = {60-71},
abstract = {Tritrichomonas foetus is a potent veterinary pathogen, causing bovine and feline trichomoniasis. The principal clinical manifestation of infection in cattle is inflammation of the genital tract and infertility. In feline, the parasite causes large-bowel disease resulting in chronic diarrhea. In contrast to other well-studied protozoan, genetic data regarding the molecular characterization and expression in T. foetus is far less understood. In this study, the first large-scale T. foetus expressed sequence tag (TfEST) project was conducted on 5064 randomly selected EST clones from a non-normalized unidirectional Tf30924 cDNA library. Assembling of 5064 single-pass sequences from the 5′ end resulted in 713 contigs and 1961 singlets. BLAST search revealed that 53.52% of the unigenes showed significant similarity to known sequences or protein motifs/domains. Functional classifications indicated that most of the unigenes are involved in translation, ribosomal structure and ribosome biogenesis. The average GC content of the T. foetus transcriptome is 40.93%. Intriguingly, only 31.29% of the unigenes contain the classical AAUAAA polyadenylation signal sequence at the 3′-UTR region. Furthermore, a panel of potential chemotherapeutic targets was also identified for the first time in T. foetus. The protein expression levels were verified by using two-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. A total of 68 highly abundant protein spots were successfully identified in the reference 2-DE map based on our T. foetus-specific protein database. The EST dataset and the reference 2-DE map established in the present study will provide a foundation for future whole genome sequencing project and comparative transcriptomic/proteomic analyses to provide potential drug targets against T. foetus infection. © 2012 Elsevier B.V.},
note = {cited By 12},
keywords = {article; expressed sequence tag; matrix assisted laser desorption ionization time of flight mass spectrometry; nonhuman; priority journal; protein domain; protein expression; protein motif; ribosome; Tritrichomonas foetus; two dimensional electrophoresis, complementary DNA; proteome; transcriptome, Electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcriptome; Tritrichomonas foetus, Two-Dimensional; Expressed Sequence Tags; Proteome; Protozoan Proteins; Spectrometry},
pubstate = {published},
tppubtype = {article}
}
Tritrichomonas foetus is a potent veterinary pathogen, causing bovine and feline trichomoniasis. The principal clinical manifestation of infection in cattle is inflammation of the genital tract and infertility. In feline, the parasite causes large-bowel disease resulting in chronic diarrhea. In contrast to other well-studied protozoan, genetic data regarding the molecular characterization and expression in T. foetus is far less understood. In this study, the first large-scale T. foetus expressed sequence tag (TfEST) project was conducted on 5064 randomly selected EST clones from a non-normalized unidirectional Tf30924 cDNA library. Assembling of 5064 single-pass sequences from the 5′ end resulted in 713 contigs and 1961 singlets. BLAST search revealed that 53.52% of the unigenes showed significant similarity to known sequences or protein motifs/domains. Functional classifications indicated that most of the unigenes are involved in translation, ribosomal structure and ribosome biogenesis. The average GC content of the T. foetus transcriptome is 40.93%. Intriguingly, only 31.29% of the unigenes contain the classical AAUAAA polyadenylation signal sequence at the 3′-UTR region. Furthermore, a panel of potential chemotherapeutic targets was also identified for the first time in T. foetus. The protein expression levels were verified by using two-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. A total of 68 highly abundant protein spots were successfully identified in the reference 2-DE map based on our T. foetus-specific protein database. The EST dataset and the reference 2-DE map established in the present study will provide a foundation for future whole genome sequencing project and comparative transcriptomic/proteomic analyses to provide potential drug targets against T. foetus infection. © 2012 Elsevier B.V.