2013
Huang, K. -Y.; Shin, J. -W.; Huang, P. -J.; Ku, F. -M.; Lin, W. -C.; Lin, R.; Hsu, W. -M.; Tang, P.
Functional profiling of the Tritrichomonas foetus transcriptome and proteome Journal Article
In: Molecular and Biochemical Parasitology, 187 (1), pp. 60-71, 2013, ISSN: 01666851, (cited By 12).
Abstract | Links | BibTeX | 標籤: article; expressed sequence tag; matrix assisted laser desorption ionization time of flight mass spectrometry; nonhuman; priority journal; protein domain; protein expression; protein motif; ribosome; Tritrichomonas foetus; two dimensional electrophoresis, complementary DNA; proteome; transcriptome, Electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcriptome; Tritrichomonas foetus, Two-Dimensional; Expressed Sequence Tags; Proteome; Protozoan Proteins; Spectrometry
@article{Huang201360,
title = {Functional profiling of the Tritrichomonas foetus transcriptome and proteome},
author = {K. -Y. Huang and J. -W. Shin and P. -J. Huang and F. -M. Ku and W. -C. Lin and R. Lin and W. -M. Hsu and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873300752&doi=10.1016%2fj.molbiopara.2012.12.001&partnerID=40&md5=3d7c67beac54bc68da0563a156eb09ea},
doi = {10.1016/j.molbiopara.2012.12.001},
issn = {01666851},
year = {2013},
date = {2013-01-01},
journal = {Molecular and Biochemical Parasitology},
volume = {187},
number = {1},
pages = {60-71},
abstract = {Tritrichomonas foetus is a potent veterinary pathogen, causing bovine and feline trichomoniasis. The principal clinical manifestation of infection in cattle is inflammation of the genital tract and infertility. In feline, the parasite causes large-bowel disease resulting in chronic diarrhea. In contrast to other well-studied protozoan, genetic data regarding the molecular characterization and expression in T. foetus is far less understood. In this study, the first large-scale T. foetus expressed sequence tag (TfEST) project was conducted on 5064 randomly selected EST clones from a non-normalized unidirectional Tf30924 cDNA library. Assembling of 5064 single-pass sequences from the 5′ end resulted in 713 contigs and 1961 singlets. BLAST search revealed that 53.52% of the unigenes showed significant similarity to known sequences or protein motifs/domains. Functional classifications indicated that most of the unigenes are involved in translation, ribosomal structure and ribosome biogenesis. The average GC content of the T. foetus transcriptome is 40.93%. Intriguingly, only 31.29% of the unigenes contain the classical AAUAAA polyadenylation signal sequence at the 3′-UTR region. Furthermore, a panel of potential chemotherapeutic targets was also identified for the first time in T. foetus. The protein expression levels were verified by using two-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. A total of 68 highly abundant protein spots were successfully identified in the reference 2-DE map based on our T. foetus-specific protein database. The EST dataset and the reference 2-DE map established in the present study will provide a foundation for future whole genome sequencing project and comparative transcriptomic/proteomic analyses to provide potential drug targets against T. foetus infection. © 2012 Elsevier B.V.},
note = {cited By 12},
keywords = {article; expressed sequence tag; matrix assisted laser desorption ionization time of flight mass spectrometry; nonhuman; priority journal; protein domain; protein expression; protein motif; ribosome; Tritrichomonas foetus; two dimensional electrophoresis, complementary DNA; proteome; transcriptome, Electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcriptome; Tritrichomonas foetus, Two-Dimensional; Expressed Sequence Tags; Proteome; Protozoan Proteins; Spectrometry},
pubstate = {published},
tppubtype = {article}
}
2012
Huang, K-Y.; Huang, P. -J.; Ku, F. -M.; Lin, R.; Alderete, J. F.; Tanga, P.
Comparative transcriptomic and proteomic analyses of Trichomonas vaginalis following adherence to fibronectin Journal Article
In: Infection and Immunity, 80 (11), pp. 3900-3911, 2012, ISSN: 00199567, (cited By 27).
Abstract | Links | BibTeX | 標籤: article; cell adhesion; gene expression profiling; gene library; nonhuman; priority journal; protein binding; protein expression; proteomics; sequence analysis; transcriptomics; Trichomonas vaginalis; trophozoite; upregulation, Cell Adhesion; Electrophoresis, complementary DNA; cysteine proteinase; fibronectin; glyceraldehyde 3 phosphate; glyceraldehyde 3 phosphate dehydrogenase; heat shock protein, Gel, Messenger; Signal Transduction; Transcriptome; Trichomonas vaginalis, Two-Dimensional; Fibronectins; Gene Expression Profiling; Mass Spectrometry; Proteome; Proteomics; Protozoan Proteins; Real-Time Polymerase Chain Reaction; RNA
@article{Huang20123900,
title = {Comparative transcriptomic and proteomic analyses of Trichomonas vaginalis following adherence to fibronectin},
author = {K-Y. Huang and P. -J. Huang and F. -M. Ku and R. Lin and J. F. Alderete and P. Tanga},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867615951&doi=10.1128%2fIAI.00611-12&partnerID=40&md5=9ea1cc7a4bea8c489b9183b798b74289},
doi = {10.1128/IAI.00611-12},
issn = {00199567},
year = {2012},
date = {2012-01-01},
journal = {Infection and Immunity},
volume = {80},
number = {11},
pages = {3900-3911},
abstract = {The morphological transformation of Trichomonas vaginalis from an ellipsoid form in batch culture to an adherent amoeboid form results from the contact of parasites with vaginal epithelial cells and with immobilized fibronectin (FN), a basement membrane component. This suggests host signaling of the parasite. We applied integrated transcriptomic and proteomic approaches to investigate the molecular responses of T. vaginalis upon binding to FN. A transcriptome analysis was performed by using large-scale expressed-sequence-tag (EST) sequencing. A total of 20,704 ESTs generated from batch culture (trophozoite-EST) versus FN-amoeboid trichomonad (FN-EST) cDNA libraries were analyzed. The FN-EST library revealed decreased amounts of transcripts that were of lower abundance in the trophozoite-EST library. There was a shift by FN-bound organisms to the expression of transcripts encoding essential proteins, possibly indicating the expression of genes for adaptation to the morphological changes needed for the FN-adhesive processes. In addition, we identified 43 differentially expressed proteins in the proteomes of FN-bound and unbound trichomonads. Among these proteins, cysteine peptidase, glyceraldehyde-3-phosphate dehydrogenase (an FN-binding protein), and stress-related proteins were upregulated in the FN-adherent cells. Stress-related genes and proteins were highly expressed in both the transcriptome and proteome of FN-bound organisms, implying that these genes and proteins may play critical roles in the response to adherence. This is the first report of a comparative proteomic and transcriptomic analysis after the binding of T. vaginalis to FN. This approach may lead to the discovery of novel virulence genes and affirm the role of genes involved in disease pathogenesis. This knowledge will permit a greater understanding of the complex host-parasite interplay. © 2012, American Society for Microbiology.},
note = {cited By 27},
keywords = {article; cell adhesion; gene expression profiling; gene library; nonhuman; priority journal; protein binding; protein expression; proteomics; sequence analysis; transcriptomics; Trichomonas vaginalis; trophozoite; upregulation, Cell Adhesion; Electrophoresis, complementary DNA; cysteine proteinase; fibronectin; glyceraldehyde 3 phosphate; glyceraldehyde 3 phosphate dehydrogenase; heat shock protein, Gel, Messenger; Signal Transduction; Transcriptome; Trichomonas vaginalis, Two-Dimensional; Fibronectins; Gene Expression Profiling; Mass Spectrometry; Proteome; Proteomics; Protozoan Proteins; Real-Time Polymerase Chain Reaction; RNA},
pubstate = {published},
tppubtype = {article}
}
2009
Huang, K. -Y.; Chien, K. -Y.; Lin, Y. -C.; Hsu, W. -M.; Fong, I. -K.; Huang, P. -J.; Yueh, Y. -M.; Gan, R. R. -C.; Tang, P.
A proteome reference map of Trichomonas vaginalis Journal Article
In: Parasitology Research, 104 (4), pp. 927-933, 2009, ISSN: 09320113, (cited By 32).
Abstract | Links | BibTeX | 標籤: Animals; Databases, Computer-Assisted; Proteome; Proteomics; Protozoan Proteins; Reference Standards; Spectrometry, cysteine proteinase; cytoskeleton protein; proteome; proteome; protozoal protein, development and aging; human; image processing; mass spectrometry; metabolism; protein database; proteomics; standard; trophozoite; two dimensional gel electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Trichomonas vaginalis; Trophozoites, Protein; Electrophoresis, Protozoa; Trichomonas vaginalis, Two-Dimensional; Female; Gene Expression Profiling; Humans; Image Processing
@article{Huang2009927,
title = {A proteome reference map of Trichomonas vaginalis},
author = {K. -Y. Huang and K. -Y. Chien and Y. -C. Lin and W. -M. Hsu and I. -K. Fong and P. -J. Huang and Y. -M. Yueh and R. R. -C. Gan and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-65549127214&doi=10.1007%2fs00436-008-1274-z&partnerID=40&md5=7ac1b50bbb56ff86abf4050baf72eb44},
doi = {10.1007/s00436-008-1274-z},
issn = {09320113},
year = {2009},
date = {2009-01-01},
journal = {Parasitology Research},
volume = {104},
number = {4},
pages = {927-933},
abstract = {Trichomoniasis caused by Trichomonas vaginalis is the most common sexual transmitted infection in the world. The 170-MB genome of this protozoan contains 60,000 genes, the largest number of genes ever identified in protozoan. High-throughput expression sequenced tag analysis showed that at least 4,000 genes were expressed in the trophozoite stage. In the present study, we use two-dimensional electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis to profile, identify, and characterize proteins expressed in the trophozoite stage of T. vaginalis. A total of 247 spots representing 164 different proteins were identified. The identified proteins with known sequence or motif/domain homologies were further classified into groups according to their biological functions. Among them, proteins related to carbohydrate metabolism represented the most abundant category in the T. vaginalis proteome. This study presented the most extensive proteomic analysis of T. vaginalis to date and provided a reference proteome database for future comparative proteomic studies. © 2008 Springer-Verlag.},
note = {cited By 32},
keywords = {Animals; Databases, Computer-Assisted; Proteome; Proteomics; Protozoan Proteins; Reference Standards; Spectrometry, cysteine proteinase; cytoskeleton protein; proteome; proteome; protozoal protein, development and aging; human; image processing; mass spectrometry; metabolism; protein database; proteomics; standard; trophozoite; two dimensional gel electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Trichomonas vaginalis; Trophozoites, Protein; Electrophoresis, Protozoa; Trichomonas vaginalis, Two-Dimensional; Female; Gene Expression Profiling; Humans; Image Processing},
pubstate = {published},
tppubtype = {article}
}