2013
Huang, K. -Y.; Shin, J. -W.; Huang, P. -J.; Ku, F. -M.; Lin, W. -C.; Lin, R.; Hsu, W. -M.; Tang, P.
Functional profiling of the Tritrichomonas foetus transcriptome and proteome Journal Article
In: Molecular and Biochemical Parasitology, 187 (1), pp. 60-71, 2013, ISSN: 01666851, (cited By 12).
Abstract | Links | BibTeX | 標籤: article; expressed sequence tag; matrix assisted laser desorption ionization time of flight mass spectrometry; nonhuman; priority journal; protein domain; protein expression; protein motif; ribosome; Tritrichomonas foetus; two dimensional electrophoresis, complementary DNA; proteome; transcriptome, Electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcriptome; Tritrichomonas foetus, Two-Dimensional; Expressed Sequence Tags; Proteome; Protozoan Proteins; Spectrometry
@article{Huang201360,
title = {Functional profiling of the Tritrichomonas foetus transcriptome and proteome},
author = {K. -Y. Huang and J. -W. Shin and P. -J. Huang and F. -M. Ku and W. -C. Lin and R. Lin and W. -M. Hsu and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873300752&doi=10.1016%2fj.molbiopara.2012.12.001&partnerID=40&md5=3d7c67beac54bc68da0563a156eb09ea},
doi = {10.1016/j.molbiopara.2012.12.001},
issn = {01666851},
year = {2013},
date = {2013-01-01},
journal = {Molecular and Biochemical Parasitology},
volume = {187},
number = {1},
pages = {60-71},
abstract = {Tritrichomonas foetus is a potent veterinary pathogen, causing bovine and feline trichomoniasis. The principal clinical manifestation of infection in cattle is inflammation of the genital tract and infertility. In feline, the parasite causes large-bowel disease resulting in chronic diarrhea. In contrast to other well-studied protozoan, genetic data regarding the molecular characterization and expression in T. foetus is far less understood. In this study, the first large-scale T. foetus expressed sequence tag (TfEST) project was conducted on 5064 randomly selected EST clones from a non-normalized unidirectional Tf30924 cDNA library. Assembling of 5064 single-pass sequences from the 5′ end resulted in 713 contigs and 1961 singlets. BLAST search revealed that 53.52% of the unigenes showed significant similarity to known sequences or protein motifs/domains. Functional classifications indicated that most of the unigenes are involved in translation, ribosomal structure and ribosome biogenesis. The average GC content of the T. foetus transcriptome is 40.93%. Intriguingly, only 31.29% of the unigenes contain the classical AAUAAA polyadenylation signal sequence at the 3′-UTR region. Furthermore, a panel of potential chemotherapeutic targets was also identified for the first time in T. foetus. The protein expression levels were verified by using two-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. A total of 68 highly abundant protein spots were successfully identified in the reference 2-DE map based on our T. foetus-specific protein database. The EST dataset and the reference 2-DE map established in the present study will provide a foundation for future whole genome sequencing project and comparative transcriptomic/proteomic analyses to provide potential drug targets against T. foetus infection. © 2012 Elsevier B.V.},
note = {cited By 12},
keywords = {article; expressed sequence tag; matrix assisted laser desorption ionization time of flight mass spectrometry; nonhuman; priority journal; protein domain; protein expression; protein motif; ribosome; Tritrichomonas foetus; two dimensional electrophoresis, complementary DNA; proteome; transcriptome, Electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcriptome; Tritrichomonas foetus, Two-Dimensional; Expressed Sequence Tags; Proteome; Protozoan Proteins; Spectrometry},
pubstate = {published},
tppubtype = {article}
}
2009
Huang, K. -Y.; Chien, K. -Y.; Lin, Y. -C.; Hsu, W. -M.; Fong, I. -K.; Huang, P. -J.; Yueh, Y. -M.; Gan, R. R. -C.; Tang, P.
A proteome reference map of Trichomonas vaginalis Journal Article
In: Parasitology Research, 104 (4), pp. 927-933, 2009, ISSN: 09320113, (cited By 32).
Abstract | Links | BibTeX | 標籤: Animals; Databases, Computer-Assisted; Proteome; Proteomics; Protozoan Proteins; Reference Standards; Spectrometry, cysteine proteinase; cytoskeleton protein; proteome; proteome; protozoal protein, development and aging; human; image processing; mass spectrometry; metabolism; protein database; proteomics; standard; trophozoite; two dimensional gel electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Trichomonas vaginalis; Trophozoites, Protein; Electrophoresis, Protozoa; Trichomonas vaginalis, Two-Dimensional; Female; Gene Expression Profiling; Humans; Image Processing
@article{Huang2009927,
title = {A proteome reference map of Trichomonas vaginalis},
author = {K. -Y. Huang and K. -Y. Chien and Y. -C. Lin and W. -M. Hsu and I. -K. Fong and P. -J. Huang and Y. -M. Yueh and R. R. -C. Gan and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-65549127214&doi=10.1007%2fs00436-008-1274-z&partnerID=40&md5=7ac1b50bbb56ff86abf4050baf72eb44},
doi = {10.1007/s00436-008-1274-z},
issn = {09320113},
year = {2009},
date = {2009-01-01},
journal = {Parasitology Research},
volume = {104},
number = {4},
pages = {927-933},
abstract = {Trichomoniasis caused by Trichomonas vaginalis is the most common sexual transmitted infection in the world. The 170-MB genome of this protozoan contains 60,000 genes, the largest number of genes ever identified in protozoan. High-throughput expression sequenced tag analysis showed that at least 4,000 genes were expressed in the trophozoite stage. In the present study, we use two-dimensional electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis to profile, identify, and characterize proteins expressed in the trophozoite stage of T. vaginalis. A total of 247 spots representing 164 different proteins were identified. The identified proteins with known sequence or motif/domain homologies were further classified into groups according to their biological functions. Among them, proteins related to carbohydrate metabolism represented the most abundant category in the T. vaginalis proteome. This study presented the most extensive proteomic analysis of T. vaginalis to date and provided a reference proteome database for future comparative proteomic studies. © 2008 Springer-Verlag.},
note = {cited By 32},
keywords = {Animals; Databases, Computer-Assisted; Proteome; Proteomics; Protozoan Proteins; Reference Standards; Spectrometry, cysteine proteinase; cytoskeleton protein; proteome; proteome; protozoal protein, development and aging; human; image processing; mass spectrometry; metabolism; protein database; proteomics; standard; trophozoite; two dimensional gel electrophoresis, Gel, Mass, Matrix-Assisted Laser Desorption-Ionization; Trichomonas vaginalis; Trophozoites, Protein; Electrophoresis, Protozoa; Trichomonas vaginalis, Two-Dimensional; Female; Gene Expression Profiling; Humans; Image Processing},
pubstate = {published},
tppubtype = {article}
}