2012
Huang, P. -J.; Lin, W. -C.; Chen, S. -C.; Lin, Y. -H.; Sun, C. -H.; Lyu, P. -C.; Tang, P.
Identification of putative miRNAs from the deep-branching unicellular flagellates Journal Article
In: Genomics, 99 (2), pp. 101-107, 2012, ISSN: 08887543, (cited By 29).
Abstract | Links | BibTeX | 標籤: Animalia; Eukaryota; Giardia intestinalis; Mastigophora (flagellates); Pentatrichomonas hominis; Protista; Trichomonas vaginalis; Tritrichomonas foetus, article; bioinformatics; controlled study; flagellate; gene control; gene expression profiling; Giardia lamblia; high throughput sequencing; nonhuman; priority journal; reverse transcription polymerase chain reaction; trichomonas hominis; Trichomonas vaginalis; Tritrichomonas foetus; trophozoite, Chromosome Mapping; Gene Expression Profiling; Giardia lamblia; High-Throughput Nucleotide Sequencing; MicroRNAs; Real-Time Polymerase Chain Reaction; RNA, DNA; Trichomonadida, microRNA, Protozoan; Sequence Analysis
@article{Huang2012101,
title = {Identification of putative miRNAs from the deep-branching unicellular flagellates},
author = {P. -J. Huang and W. -C. Lin and S. -C. Chen and Y. -H. Lin and C. -H. Sun and P. -C. Lyu and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84856467364&doi=10.1016%2fj.ygeno.2011.11.002&partnerID=40&md5=5d802d8ceb2c5f478016ce37e4b904e1},
doi = {10.1016/j.ygeno.2011.11.002},
issn = {08887543},
year = {2012},
date = {2012-01-01},
journal = {Genomics},
volume = {99},
number = {2},
pages = {101-107},
abstract = {MicroRNAs (miRNAs) are a class of extensively studied RNAi-associated small RNAs that play a critical role in eukaryotic gene regulation. However, knowledge on the miRNA and its regulation in unicellular eukaryotes is very limited. In order to obtain a better understanding on the origin of miRNA regulation system, we used deep-sequencing technology to investigate the miRNA expression pattern in four deep-branching unicellular flagellates: Giardia lamblia, Trichomonas vaginalis, Tritrichomonas foetus, and Pentatrichomonas hominis. In addition to the known miRNAs that have been described in G. lamblia and T. vaginalis, we identified 14 ancient animal miRNA families and 13 plant-specific families. Bioinformatics analysis also identified four novel miRNA candidates with reliable precursor structures derived from mature tRNAs. Our results indicated that miRNAs are likely to be a general feature for gene regulation throughout unicellular and multicellular eukaryotes and some of them may derive from unconventional ncRNAs such as snoRNA and tRNA. © 2011 Elsevier Inc.},
note = {cited By 29},
keywords = {Animalia; Eukaryota; Giardia intestinalis; Mastigophora (flagellates); Pentatrichomonas hominis; Protista; Trichomonas vaginalis; Tritrichomonas foetus, article; bioinformatics; controlled study; flagellate; gene control; gene expression profiling; Giardia lamblia; high throughput sequencing; nonhuman; priority journal; reverse transcription polymerase chain reaction; trichomonas hominis; Trichomonas vaginalis; Tritrichomonas foetus; trophozoite, Chromosome Mapping; Gene Expression Profiling; Giardia lamblia; High-Throughput Nucleotide Sequencing; MicroRNAs; Real-Time Polymerase Chain Reaction; RNA, DNA; Trichomonadida, microRNA, Protozoan; Sequence Analysis},
pubstate = {published},
tppubtype = {article}
}
2009
Lin, W. -C.; Li, S. -C.; Lin, W. -C.; Shin, J. -W.; Hu, S. -N.; Yu, X. -M.; Huang, T. -Y.; Chen, S. -C.; Chen, H. -C.; Chen, S. -J.; Huang, P. -J.; Gan, R. Ruei-Chi; Chiu, C. -H.; Tang, P.
Identification of microRNA in the protist Trichomonas vaginalis Journal Article
In: Genomics, 93 (5), pp. 487-493, 2009, ISSN: 08887543, (cited By 59).
Abstract | Links | BibTeX | 標籤: Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping; Cloning, article; bioinformatics; gene expression; gene identification; molecular cloning; nonhuman; nucleotide sequence; parasite cultivation; priority journal; protist life cycle stage; real time polymerase chain reaction; sequence homology; serial analysis of gene expression; Trichomonas vaginalis, microRNA, Molecular; Gene Expression Regulation; Genome, Protista; Trichomonas vaginalis, Protozoan; MicroRNAs; Molecular Sequence Data; Nucleic Acid Conformation; Trichomonas vaginalis
@article{Lin2009487,
title = {Identification of microRNA in the protist Trichomonas vaginalis},
author = {W. -C. Lin and S. -C. Li and W. -C. Lin and J. -W. Shin and S. -N. Hu and X. -M. Yu and T. -Y. Huang and S. -C. Chen and H. -C. Chen and S. -J. Chen and P. -J. Huang and R. Ruei-Chi Gan and C. -H. Chiu and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-64149117818&doi=10.1016%2fj.ygeno.2009.01.004&partnerID=40&md5=6502fda9f21e04ea203a95b955cef341},
doi = {10.1016/j.ygeno.2009.01.004},
issn = {08887543},
year = {2009},
date = {2009-01-01},
journal = {Genomics},
volume = {93},
number = {5},
pages = {487-493},
abstract = {MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. However, miRNA has never been identified experimentally in protist. Direct cloning of 438 expressed miRNA tags by microRNA serial analysis of gene expression from the parasitic protist Trichomonas vaginalis identified nine candidate miRNAs. Bioinformatics analysis of the corresponding genomic region revealed that these miRNA candidates contain a classical stem-loop-stem structure of pre-microRNAs. Analysis of the 20 nt long mature tva-miR-001 showed that it is an intergenic miRNA located at the scaffold DS113596. Tva-miR-001 was differentially expressed in the trophozoite, pseudocyst and amoeboid stages. Based on the experimental results of the present study, we provided solid evidence that protist possesses a miRNA regulating network comparable with multicellular organisms for the first time. © 2009 Elsevier Inc. All rights reserved.},
note = {cited By 59},
keywords = {Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping; Cloning, article; bioinformatics; gene expression; gene identification; molecular cloning; nonhuman; nucleotide sequence; parasite cultivation; priority journal; protist life cycle stage; real time polymerase chain reaction; sequence homology; serial analysis of gene expression; Trichomonas vaginalis, microRNA, Molecular; Gene Expression Regulation; Genome, Protista; Trichomonas vaginalis, Protozoan; MicroRNAs; Molecular Sequence Data; Nucleic Acid Conformation; Trichomonas vaginalis},
pubstate = {published},
tppubtype = {article}
}