2012
Huang, P. -J.; Lin, W. -C.; Chen, S. -C.; Lin, Y. -H.; Sun, C. -H.; Lyu, P. -C.; Tang, P.
Identification of putative miRNAs from the deep-branching unicellular flagellates Journal Article
In: Genomics, 99 (2), pp. 101-107, 2012, ISSN: 08887543, (cited By 29).
Abstract | Links | BibTeX | 標籤: Animalia; Eukaryota; Giardia intestinalis; Mastigophora (flagellates); Pentatrichomonas hominis; Protista; Trichomonas vaginalis; Tritrichomonas foetus, article; bioinformatics; controlled study; flagellate; gene control; gene expression profiling; Giardia lamblia; high throughput sequencing; nonhuman; priority journal; reverse transcription polymerase chain reaction; trichomonas hominis; Trichomonas vaginalis; Tritrichomonas foetus; trophozoite, Chromosome Mapping; Gene Expression Profiling; Giardia lamblia; High-Throughput Nucleotide Sequencing; MicroRNAs; Real-Time Polymerase Chain Reaction; RNA, DNA; Trichomonadida, microRNA, Protozoan; Sequence Analysis
@article{Huang2012101,
title = {Identification of putative miRNAs from the deep-branching unicellular flagellates},
author = {P. -J. Huang and W. -C. Lin and S. -C. Chen and Y. -H. Lin and C. -H. Sun and P. -C. Lyu and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84856467364&doi=10.1016%2fj.ygeno.2011.11.002&partnerID=40&md5=5d802d8ceb2c5f478016ce37e4b904e1},
doi = {10.1016/j.ygeno.2011.11.002},
issn = {08887543},
year = {2012},
date = {2012-01-01},
journal = {Genomics},
volume = {99},
number = {2},
pages = {101-107},
abstract = {MicroRNAs (miRNAs) are a class of extensively studied RNAi-associated small RNAs that play a critical role in eukaryotic gene regulation. However, knowledge on the miRNA and its regulation in unicellular eukaryotes is very limited. In order to obtain a better understanding on the origin of miRNA regulation system, we used deep-sequencing technology to investigate the miRNA expression pattern in four deep-branching unicellular flagellates: Giardia lamblia, Trichomonas vaginalis, Tritrichomonas foetus, and Pentatrichomonas hominis. In addition to the known miRNAs that have been described in G. lamblia and T. vaginalis, we identified 14 ancient animal miRNA families and 13 plant-specific families. Bioinformatics analysis also identified four novel miRNA candidates with reliable precursor structures derived from mature tRNAs. Our results indicated that miRNAs are likely to be a general feature for gene regulation throughout unicellular and multicellular eukaryotes and some of them may derive from unconventional ncRNAs such as snoRNA and tRNA. © 2011 Elsevier Inc.},
note = {cited By 29},
keywords = {Animalia; Eukaryota; Giardia intestinalis; Mastigophora (flagellates); Pentatrichomonas hominis; Protista; Trichomonas vaginalis; Tritrichomonas foetus, article; bioinformatics; controlled study; flagellate; gene control; gene expression profiling; Giardia lamblia; high throughput sequencing; nonhuman; priority journal; reverse transcription polymerase chain reaction; trichomonas hominis; Trichomonas vaginalis; Tritrichomonas foetus; trophozoite, Chromosome Mapping; Gene Expression Profiling; Giardia lamblia; High-Throughput Nucleotide Sequencing; MicroRNAs; Real-Time Polymerase Chain Reaction; RNA, DNA; Trichomonadida, microRNA, Protozoan; Sequence Analysis},
pubstate = {published},
tppubtype = {article}
}