2009
Lin, W. -C.; Huang, K. -Y.; Chen, S. -C.; Huang, T. -Y.; Chen, S. -J.; Huang, P. -J.; Tang, P.
Malate dehydrogenase is negatively regulated by miR-1 in Trichomonas vaginalis Journal Article
In: Parasitology Research, 105 (6), pp. 1683-1689, 2009, ISSN: 09320113, (cited By 16).
Abstract | Links | BibTeX | 標籤: Animals; Base Sequence; Down-Regulation; Gene Expression Profiling; Gene Expression Regulation; Malate Dehydrogenase; MicroRNAs; Molecular Sequence Data; Proteome; Trichomonas vaginalis, article; bioinformatics; controlled study; enzyme regulation; gene; gene control; genetic transfection; miR 1 gene; nonhuman; nucleotide sequence; priority journal; protein targeting; proteomics; protozoal genetics; transcriptomics; Trichomonas vaginalis; trophozoite; tv mdh gene, malate dehydrogenase; microRNA, Protista; Trichomonas vaginalis
@article{Lin20091683,
title = {Malate dehydrogenase is negatively regulated by miR-1 in Trichomonas vaginalis},
author = {W. -C. Lin and K. -Y. Huang and S. -C. Chen and T. -Y. Huang and S. -J. Chen and P. -J. Huang and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-70350228364&doi=10.1007%2fs00436-009-1616-5&partnerID=40&md5=b8fc13f71ebfc0dae3ff035018be71c2},
doi = {10.1007/s00436-009-1616-5},
issn = {09320113},
year = {2009},
date = {2009-01-01},
journal = {Parasitology Research},
volume = {105},
number = {6},
pages = {1683-1689},
abstract = {MicroRNAs are highly conserved small noncoding RNAs that can suppress protein translation through complementary binding to target mRNAs. We used a novel approach to identify miRNA targets in the protist Trichomonas vaginalis by comparing the levels of differentially expressed proteins and genes in the trophozoite and amoeboid stages. We observed that the T. vaginalis malate dehydrogenase (Tv-MDH) gene was upregulated 20-fold in the amoeboid stage, but the protein level was reduced by 4.5-fold. Bioinformatics analysis revealed that the Tv-MDH mRNA contains putative target sites of the miR-1 family. The expression level of endogenous tva-miR-1 in the amoeboid stage was 50-fold higher than in the trophozoite stage. Transfection of trophozoites with tva-miR-1 mimics reduced Tv-MDH protein expression by 60%. Based on these experimental data, we conclude that Tv-MDH is negatively regulated by tva-miR-1. The results of this study demonstrate that a combination of proteomic and transcriptomic approaches is a powerful tool for identifying miRNA targets. © 2009 Springer-Verlag.},
note = {cited By 16},
keywords = {Animals; Base Sequence; Down-Regulation; Gene Expression Profiling; Gene Expression Regulation; Malate Dehydrogenase; MicroRNAs; Molecular Sequence Data; Proteome; Trichomonas vaginalis, article; bioinformatics; controlled study; enzyme regulation; gene; gene control; genetic transfection; miR 1 gene; nonhuman; nucleotide sequence; priority journal; protein targeting; proteomics; protozoal genetics; transcriptomics; Trichomonas vaginalis; trophozoite; tv mdh gene, malate dehydrogenase; microRNA, Protista; Trichomonas vaginalis},
pubstate = {published},
tppubtype = {article}
}