2009
Lin, W. -C.; Huang, K. -Y.; Chen, S. -C.; Huang, T. -Y.; Chen, S. -J.; Huang, P. -J.; Tang, P.
Malate dehydrogenase is negatively regulated by miR-1 in Trichomonas vaginalis Journal Article
In: Parasitology Research, 105 (6), pp. 1683-1689, 2009, ISSN: 09320113, (cited By 16).
Abstract | Links | BibTeX | 標籤: Animals; Base Sequence; Down-Regulation; Gene Expression Profiling; Gene Expression Regulation; Malate Dehydrogenase; MicroRNAs; Molecular Sequence Data; Proteome; Trichomonas vaginalis, article; bioinformatics; controlled study; enzyme regulation; gene; gene control; genetic transfection; miR 1 gene; nonhuman; nucleotide sequence; priority journal; protein targeting; proteomics; protozoal genetics; transcriptomics; Trichomonas vaginalis; trophozoite; tv mdh gene, malate dehydrogenase; microRNA, Protista; Trichomonas vaginalis
@article{Lin20091683,
title = {Malate dehydrogenase is negatively regulated by miR-1 in Trichomonas vaginalis},
author = {W. -C. Lin and K. -Y. Huang and S. -C. Chen and T. -Y. Huang and S. -J. Chen and P. -J. Huang and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-70350228364&doi=10.1007%2fs00436-009-1616-5&partnerID=40&md5=b8fc13f71ebfc0dae3ff035018be71c2},
doi = {10.1007/s00436-009-1616-5},
issn = {09320113},
year = {2009},
date = {2009-01-01},
journal = {Parasitology Research},
volume = {105},
number = {6},
pages = {1683-1689},
abstract = {MicroRNAs are highly conserved small noncoding RNAs that can suppress protein translation through complementary binding to target mRNAs. We used a novel approach to identify miRNA targets in the protist Trichomonas vaginalis by comparing the levels of differentially expressed proteins and genes in the trophozoite and amoeboid stages. We observed that the T. vaginalis malate dehydrogenase (Tv-MDH) gene was upregulated 20-fold in the amoeboid stage, but the protein level was reduced by 4.5-fold. Bioinformatics analysis revealed that the Tv-MDH mRNA contains putative target sites of the miR-1 family. The expression level of endogenous tva-miR-1 in the amoeboid stage was 50-fold higher than in the trophozoite stage. Transfection of trophozoites with tva-miR-1 mimics reduced Tv-MDH protein expression by 60%. Based on these experimental data, we conclude that Tv-MDH is negatively regulated by tva-miR-1. The results of this study demonstrate that a combination of proteomic and transcriptomic approaches is a powerful tool for identifying miRNA targets. © 2009 Springer-Verlag.},
note = {cited By 16},
keywords = {Animals; Base Sequence; Down-Regulation; Gene Expression Profiling; Gene Expression Regulation; Malate Dehydrogenase; MicroRNAs; Molecular Sequence Data; Proteome; Trichomonas vaginalis, article; bioinformatics; controlled study; enzyme regulation; gene; gene control; genetic transfection; miR 1 gene; nonhuman; nucleotide sequence; priority journal; protein targeting; proteomics; protozoal genetics; transcriptomics; Trichomonas vaginalis; trophozoite; tv mdh gene, malate dehydrogenase; microRNA, Protista; Trichomonas vaginalis},
pubstate = {published},
tppubtype = {article}
}
Lin, W. -C.; Li, S. -C.; Lin, W. -C.; Shin, J. -W.; Hu, S. -N.; Yu, X. -M.; Huang, T. -Y.; Chen, S. -C.; Chen, H. -C.; Chen, S. -J.; Huang, P. -J.; Gan, R. Ruei-Chi; Chiu, C. -H.; Tang, P.
Identification of microRNA in the protist Trichomonas vaginalis Journal Article
In: Genomics, 93 (5), pp. 487-493, 2009, ISSN: 08887543, (cited By 59).
Abstract | Links | BibTeX | 標籤: Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping; Cloning, article; bioinformatics; gene expression; gene identification; molecular cloning; nonhuman; nucleotide sequence; parasite cultivation; priority journal; protist life cycle stage; real time polymerase chain reaction; sequence homology; serial analysis of gene expression; Trichomonas vaginalis, microRNA, Molecular; Gene Expression Regulation; Genome, Protista; Trichomonas vaginalis, Protozoan; MicroRNAs; Molecular Sequence Data; Nucleic Acid Conformation; Trichomonas vaginalis
@article{Lin2009487,
title = {Identification of microRNA in the protist Trichomonas vaginalis},
author = {W. -C. Lin and S. -C. Li and W. -C. Lin and J. -W. Shin and S. -N. Hu and X. -M. Yu and T. -Y. Huang and S. -C. Chen and H. -C. Chen and S. -J. Chen and P. -J. Huang and R. Ruei-Chi Gan and C. -H. Chiu and P. Tang},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-64149117818&doi=10.1016%2fj.ygeno.2009.01.004&partnerID=40&md5=6502fda9f21e04ea203a95b955cef341},
doi = {10.1016/j.ygeno.2009.01.004},
issn = {08887543},
year = {2009},
date = {2009-01-01},
journal = {Genomics},
volume = {93},
number = {5},
pages = {487-493},
abstract = {MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. However, miRNA has never been identified experimentally in protist. Direct cloning of 438 expressed miRNA tags by microRNA serial analysis of gene expression from the parasitic protist Trichomonas vaginalis identified nine candidate miRNAs. Bioinformatics analysis of the corresponding genomic region revealed that these miRNA candidates contain a classical stem-loop-stem structure of pre-microRNAs. Analysis of the 20 nt long mature tva-miR-001 showed that it is an intergenic miRNA located at the scaffold DS113596. Tva-miR-001 was differentially expressed in the trophozoite, pseudocyst and amoeboid stages. Based on the experimental results of the present study, we provided solid evidence that protist possesses a miRNA regulating network comparable with multicellular organisms for the first time. © 2009 Elsevier Inc. All rights reserved.},
note = {cited By 59},
keywords = {Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping; Cloning, article; bioinformatics; gene expression; gene identification; molecular cloning; nonhuman; nucleotide sequence; parasite cultivation; priority journal; protist life cycle stage; real time polymerase chain reaction; sequence homology; serial analysis of gene expression; Trichomonas vaginalis, microRNA, Molecular; Gene Expression Regulation; Genome, Protista; Trichomonas vaginalis, Protozoan; MicroRNAs; Molecular Sequence Data; Nucleic Acid Conformation; Trichomonas vaginalis},
pubstate = {published},
tppubtype = {article}
}